Part:BBa_J44013:Design

HixC-pBAD-HixC-Tetrev-RBSrev-HixC

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 159
  Illegal NheI site found at 1249
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 99
  Illegal BamHI site found at 1103
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 549
  Illegal NgoMIV site found at 709
  Illegal NgoMIV site found at 1077
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

Trascription readthrough into part appears to have been blocked by the introduction of double forward terminators before and after the cloning site.

Source

Cloned from J44011.